Microencapsulation of growth factors by microfluidic system.
Lilith M Caballero-Aguilar, Serena Duchi, Anita Quigley, Carmine Onofrillo, Claudia Di Bella, Simon E Moulton
MethodsX | Elsevier BV | Published : 2021
The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an algin..View full abstract