Journal article
Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride
K Chandrasekar, BL Farrugia, L Johnson, D Marks, D Irving, Z Elgundi, K Lau, HN Kim, J Rnjak-Kovacina, MM Bilek, JM Whitelock, MS Lord
Advanced Healthcare Materials | WILEY | Published : 2021
Abstract
Surface modification of biomaterials is a promising approach to control biofunctionality while retaining the bulk biomaterial properties. Perlecan is the major proteoglycan in the vascular basement membrane that supports low levels of platelet adhesion but not activation. Thus, perlecan is a promising bioactive for blood-contacting applications. This study furthers the mechanistic understanding of platelet interactions with perlecan by establishing that platelets utilize domains III and V of the core protein for adhesion. Polyvinyl chloride (PVC) is functionalized with recombinant human perlecan domain V (rDV) to explore the effect of the tethering method on proteoglycan orientation and bioa..
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Awarded by Australian Research Council
Funding Acknowledgements
The authors would like to acknowledge funding support from the Australian Research Council, DP150104242 (M.S.L., J.M.W., and J.R.-K.), LP140101056 (M.S.L., J.M.W., and D.I.), and FL190100216 (M.M.B.). K.C., K.L., and H.N.K. were supported by the Australian Government Research Training Program Scholarships. J.R.-K. was supported by the Heart Foundation of Australia Future Leader Fellowship (101896). SEM analysis was performed at the Electron Microscope Unit (EMU), mass spectrometry was performed at the Bioanalytical Mass Spectrometry Facility (BMSF), and QCM-D was performed at the Molecular Surface Interaction (MSI) Network Lab. EMU and BMSF are part of the Mark Wainwright Analytical Center at UNSW Sydney, which, in addition to MSI (Z.E.), is in part funded by the Research Infrastructure program at UNSW. The authors thank Dr. Sydney Liu at BMSF for performing mass spectrometry analysis. The Australian governments fund the Australian Red Cross Lifeblood to provide blood, blood products, and services to the Australian community.