Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride.
Keerthana Chandrasekar, Brooke L Farrugia, Lacey Johnson, Denese Marks, David Irving, Zehra Elgundi, Kieran Lau, Ha Na Kim, Jelena Rnjak-Kovacina, Marcela M Bilek, John M Whitelock, Megan S Lord
Advanced Healthcare Materials | Published : 2021
Surface modification of biomaterials is a promising approach to control biofunctionality while retaining the bulk biomaterial properties. Perlecan is the major proteoglycan in the vascular basement membrane that supports low levels of platelet adhesion but not activation. Thus, perlecan is a promising bioactive for blood-contacting applications. This study furthers the mechanistic understanding of platelet interactions with perlecan by establishing that platelets utilize domains III and V of the core protein for adhesion. Polyvinyl chloride (PVC) is functionalized with recombinant human perlecan domain V (rDV) to explore the effect of the tethering method on proteoglycan orientation and bioa..View full abstract