Journal article

Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems

Federico Martinez-Seidel, Pipob Suwanchaikasem, Shuai Nie, Michael G Leeming, Alexandre Augusto Pereira Firmino, Nicholas A Williamson, Joachim Kopka, Ute Roessner, Berin A Boughton

Frontiers in Plant Science | FRONTIERS MEDIA SA | Published : 2021

Abstract

Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we repor..

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Grants

Funding Acknowledgements

PS would like to thank the Melbourne Research Scholarship and Nutrifield Pty Ltd., for financially supporting his Ph.D. program. FM-S would like to acknowledge the Max-Planck Society (Max Planck Institute ofMolecular Plant Physiology) and The University of Melbourne for funding his research via the Melbourne-Potsdam Ph.D. Programme (MelPoPP).