Journal article

Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed

Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK | Published : 2014

Abstract

Exon 2 of the Ovar-DRB1 gene of 90 Makuie sheep was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Ten alleles and 18 genotypes were identified. The frequencies of the genotypes were 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487, and 0.0365 for AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ, and AV, respectively. Allele frequencies were 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854, and 0.0183 for A, B, E, F, I, M, O, P, Q, and V, respectively. There was no significant (P = 0.05) deviation from Hardy..

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Funding Acknowledgements

This research work was supported by the Department of Animal Science and the Institute of Biotechnology, Urmia University, Urmia, Iran.