Journal article
Bioinformatic and expression analyses of genes mediating zinc homeostasis in Nostoc punctiforme
L Hudek, LC Rai, D Freestone, A Michalczyk, M Gibson, YF Song, M Leigh Ackland
Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2009
DOI: 10.1128/AEM.02481-08
Abstract
Zinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn2+ over 14 days showed that ZnCl2 levels above 22 μM significantly reduced cell viability. After 3 days in 22 μM ZnCl2, ca. 12% of the Zn2+was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 μM relative to 0 μM treatment. Radiolabeled 65Zn showed Zn2+ uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn2+ uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the pred..
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Funding Acknowledgements
[ "This study was supported by funding from the International Science Linkage Programme, Australia-China Special Fund for Scientific and Technological Cooperation.", "We are grateful to Matt Beasley, Ben R. Kiefel (Alchemy Biosciences), and Peter Beech for technical assistance and helpful discussions." ]