Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallén)

Abstract

Cytochrome P450 monooxygenase (P450)-mediated detoxification is an important mechanism involved in the resistance to neurotoxic pesticides. However, the molecular basis of the mechanism of P450s, as associated with the resistance to growth regulator insecticides (IGRs) remains largely unknown. In this study, a resistance strain (YN-BPF) of Laodelphax striatellus was developed, with 59.9-fold resistance to buprofezin, through 42 generations of discontinuous selections of the susceptible strain (YN) with buprofezin (an IGR). A synergistic study in vivo and a biochemical study in vitro indicated that an enhanced detoxification mediated by P450s to some extent contributes to the buprofezin resis..