Journal article

Direct Observation of the Dynamics of Single-Cell Metabolic Activity during Microbial Diauxic Growth

HLO McClelland, C Jones, LM Chubiz, DA Fike, AS Bradley

mBio | American Society for Microbiology | Published : 2020

Abstract

Population-level analyses are rapidly becoming inadequate to answer many of biomedical science and microbial ecology’s most pressing questions. The role of microbial populations within ecosystems and the evolutionary selective pressure on individuals depend fundamentally on the metabolic activity of single cells. Yet, many existing single-cell technologies provide only indirect evidence of metabolic specialization because they rely on correlations between transcription and phenotype established at the level of the population to infer activity. In this study, we take a top-down approach using isotope labels and secondary ion mass spectrometry to track the uptake of carbon and nitrogen atoms f..

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Grants

Awarded by DOE/BER


Funding Acknowledgements

We acknowledge funding to D.A.F. and A.S.B. from DOE/BER (grant DE-SC0014613). Conceptualization, A.S.B., D.A.F., and H.L.O.M.; methodology, H.L.O.M.; investigation, H.L.O.M., C.J., and L.M.C.; writing-original draft, H.L.O.M.; writing-review and editing, all authors; funding acquisition, A.S.B. and D.A.F.; resources, A.S.B. and D.A.F.