Journal article

Proteomic analyses reveal that immune integrins are major targets for regulation by Membrane-Associated Ring-CH (MARCH) proteins MARCH2, 3, 4 and 9

Jarrod J Sandow, Andrew I Webb, Dina Stockwell, Nadia J Kershaw, Cyrus Tan, Satoshi Ishido, Warren S Alexander, Douglas J Hilton, Jeffrey J Babon, Nicos A Nicola

PROTEOMICS | WILEY | Published : 2021

Abstract

MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that dampen immune responses by downregulating cell surface expression of major histocompatibility complexes I and II as well as immune co-stimulatory receptors. We recently showed that MARCH2,3,4 and 9 also downregulate cell surface expression of the inflammatory cytokine receptor for interleukin-6 (IL6Rα). Here we use over-expression of these MARCH proteins in the M1 myeloid leukaemia cell line and cell surface proteomic analyses to globally analyse other potential targets of these proteins. A large range of cell surface proteins regulated by more than one MARCH protein in addition to several MARCH protein-specific cell su..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by NHMRC IRISS scheme grant


Funding Acknowledgements

Janelle Lochland is thanked for genotyping mouse strains and the WEHI Bioservices facility is thanked for animal husbandry. This work was supported by grants from the National Health and Medical Research Council of Australia (Program Grant 1113577, Project Grant 1099752, and Fellowship grants to N.A.N. (1078737), W.S.A. (1058344), and J.J.B. (1121755) as well as infrastructure support from the NHMRC IRISS scheme grant 9000220 and a Victorian State Government Operational Infrastructure Scheme grant.