Journal article

Nanophotonics enhanced coverslip for phase imaging in biology

Lukas Wesemann, Jon Rickett, Jingchao Song, Jieqiong Lou, Elizabeth Hinde, Timothy J Davis, Ann Roberts

Light: Science & Applications | SPRINGERNATURE | Published : 2021

Abstract

The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all-optical generation of pseudo three-dimensional images of phase objects on transmission. We show that by placing unstained human cancer cells on the device, the internal structure within the cells can be clearly seen. Our research demonstrates the significant potential of nanophotonic devices for integration into compact imaging and medical diagnostic devices. The nanophotonics enhanced coverslip (NEC) ena..

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Grants

Awarded by Australian Research Council


Awarded by Australian Research Council Center of Excellence Scheme


Awarded by National Health and Medical Research Council Career Development Fellowship


Funding Acknowledgements

This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). We acknowledge funding through the Australian Research Council Discovery Projects Scheme (DP160100983, DP180101387) and the Center of Excellence Scheme (CE200100010). Furthermore, we acknowledge funding through the National Health and Medical Research Council Career Development Fellowship (APP1124762) as well as the Albert Shimmins Continuity research fund.