Journal article

Identification of ZBTB18 as a novel colorectal tumor suppressor gene through genome-wide promoter hypermethylation analysis

Sarah Bazzocco, Higinio Dopeso, Agueda Martinez-Barriocanal, Estefania Anguita, Rocio Nieto, Jing Li, Elia Garcia-Vidal, Valentina Maggio, Paulo Rodrigues, Priscila Guimaraes de Marcondes, Simo Schwartz, Lauri A Aaltonen, Alex Sanchez, John M Mariadason, Diego Arango



BACKGROUND: Cancer initiation and progression are driven by genetic and epigenetic changes. Although genome/exome sequencing has significantly contributed to the characterization of the genetic driver alterations, further investigation is required to systematically identify cancer driver genes regulated by promoter hypermethylation. RESULTS: Using genome-wide analysis of promoter methylation in 45 colorectal cancer cell lines, we found that higher overall methylation levels were associated with microsatellite instability (MSI), faster proliferation and absence of APC mutations. Because epigenetically silenced genes could represent important oncogenic drivers, we used mRNA expression profilin..

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University of Melbourne Researchers


Awarded by European Regional Development Fund (ERDF)

Awarded by Spanish Association Against Cancer (AECC)

Funding Acknowledgements

This study was partially funded by grants of the Instituto de Salud Carlos III and the European Regional Development Fund (ERDF; PI16/00540, PI19/00993, AC20/00022 and AC15/00066) and the Spanish Association Against Cancer (AECC GCA15152966ARAN) to Diego Arango.