Journal article

Evaluation of the Roche MagNA Pure 96 nucleic acid extraction platform for the Seegene Anyplex II HPV28 detection assay

S Atchison, H Shilling, P Balgovind, DA Machalek, D Hawkes, SM Garland, M Saville, G Murray, M Molano, J Danielewski, S Phillips

JOURNAL OF APPLIED MICROBIOLOGY | WILEY | Published : 2021

Abstract

AIM: Validate the Roche, MagNAPure96 (MP96) nucleic acid extraction platform for Seegene Anyplex II HPV28 (Anyplex28) detection of Human Papillomavirus. METHODS AND RESULTS: Comparisons were made for Anyplex28 genotyping from 115 cervical samples extracted on the Hamilton, STARlet and the MP96. Two DNA concentrations were used for the MP96, one matched for sample input to the STARlet and another 5× concentration (laboratory standard). Agreement of HPV detection was 89·8% (κ = 0·798; P = 0·007), with HPV detected in 10 more samples for the MP96. There was a high concordance of detection for any oncogenic HPV genotype (κ = 0·77; P = 0·007) and for any low-risk HPV genotype (κ = 0·85; P = 0·008..

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Grants

Awarded by Commonwealth Department of Health HPV Surveillance Fund


Funding Acknowledgements

This work is supported by the Commonwealth Department of Health HPV Surveillance Fund (Activity number 4-8FG4IWD). The funders had no role in study design, data collection, analyses, and interpretation of data, writing of the report, or in the decision to submit the article for publication.