Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis
Vinzenz Hofferek, Huaqi Su, Gavin E Reid, FF Hsu (ed.)
MASS SPECTROMETRY-BASED LIPIDOMICS | HUMANA PRESS INC | Published : 2021
Chemical derivatization coupled with nano-electrospray ionization (nESI) and ultra-high resolution accurate mass spectrometry (UHRAMS) is an established approach to overcome isobaric and isomeric mass interference limitations, and improve the analytical performance, of direct-infusion (i.e., "shotgun") lipidome analysis strategies for "sum composition" level identification and quantification of individual lipid species from within complex mixtures. Here, we describe a protocol for sequential functional group selective derivatization of aminophospholipids and O-alk-1'-enyl (i.e., plasmalogen) lipids, that when integrated into a shotgun lipidomics workflow involving deuterium-labeled internal ..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC)
Awarded by Australian Research Council (ARC)
This work was supported by research grants APP1156778 and APP1142750 from the National Health and Medical Research Council (NHMRC), and research grant DP190102464 from the Australian Research Council (ARC).