Journal article

Molecular phylogeny of tribe Myoporeae (Scrophulariaceae) using nuclear ribosomal DNA: Generic relationships and evidence for major clades

Rachael M Fowler, Daniel J Murphy, Todd GB McLay, Bevan J Buirchell, Robert J Chinnock, Michael J Bayly

TAXON | WILEY | Published : 2021


Eremophila is a significant component of the Australian arid zone flora, but its generic limits and relationships to the other six genera of tribe Myoporeae remain largely untested. In this study, we assembled a dataset of the nuclear ribosomal cistron (ca. 6000 bases including ITS1+2, ETS regions, non-transcribed spacer and associated genes) for all genera in tribe Myoporeae with a particular focus on Eremophila (205 of total 233 formally described species of Eremophila, and 28 species of related genera sampled). Phylogenetic analyses were performed using maximum likelihood and Bayesian inference methods. These resolved four major clades that were well supported. Eremophila was paraphyletic..

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Awarded by Novo Nordisk Foundation Interdisciplinary Synergy Programme

Funding Acknowledgements

We would like to thank Peter Hall, Rob Davis, Tanja Schuster, Trevor Wilson, Neil Gibson and Russell Waite for numerous collections of Eremophila from right across Australia. We would also like to thank Ron and Claire Dadd for allowing access to their extensive Eremophila garden collection and the AustralianNational Botanic Garden (Canberra), Royal Botanic Gardens Victoria (Melbourne), Botanic Gardens of South Australia and State Herbarium (Adelaide), Australian Arid Lands Botanic Garden (Port Augusta), Olive Pink Botanic Garden (Alice Springs), Kings Park and Botanic Garden (Perth) and Norfolk Island National Park for access to living collections. For international collections we would like to thank Jen Tate fromMassey University of New Zealand for the collection of Myoporum laetum, Marilyn Griffiths from Fairchild Tropical Botanic Garden for the collection of Bontia daphnoides, ChiouRong Sheue and Dr Tang from National Chung Hsing University for the collection of Pentacoelium bontioides, David Lorence from the National Tropical Botanical Garden, Hawaii for the collection of Myoporum sandwicense and Jason Cantley and Tim Gallaher for the supply of numerous non-Australian species of Myoporum. We would also like to thank Josquin Tibbits for technical assistance when preparing DNA libraries, and Stephen Wilcox and Liz Milla for their assistance in sequencing DNA libraries. This research was completed as part of a Ph.D. study by RMF and was funded by a Jim Ross Ph.D. Studentship (Cybec Foundation in conjunction with Royal Botanic Gardens Victoria), Helen McLellan Research Grant, Australian Conservation Taxonomy Award from The Nature Conservancy and Australasian Systematic Botany Society, Alfred Nicholas Fellowship from The University of Melbourne, and the Novo Nordisk Foundation Interdisciplinary Synergy Programme (grant no. NNF16OC0021616).