Gene expression profiling of reproductive meristem types in early rice inflorescences by laser microdissection
Thomas WR Harrop, Israr Ud Din, Veronica Gregis, Michela Osnato, Stefan Jouannic, Helene Adam, Martin M Kater
PLANT JOURNAL | WILEY | Published : 2016
In rice, inflorescence architecture is established at early stages of reproductive development and contributes directly to grain yield potential. After induction of flowering, the complexity of branching, and therefore the number of seeds on the panicle, is determined by the activity of different meristem types and the timing of transitions between them. Although some of the genes involved in these transitions have been identified, an understanding of the network of transcriptional regulators controlling this process is lacking. To address this we used a precise laser microdissection and RNA-sequencing approach in Oryza sativa ssp. japonica cv. Nipponbare to produce quantitative data that de..View full abstract
Awarded by Fondation Agropolis through the Investissements d'avenir programme
Awarded by Fondazione Cariplo
We thank James Tregear for feedback and discussion, and Jean-Luc Verdeil and Genevieve Conejero for assistance with laser microdissection at the PHIV imaging platform (CIRAD, Montpellier, France). This project was jointly supported by Fondation Agropolis through the Investissements d'avenir programme (ANR-10-LABX-0001-01) and Fondazione Cariplo under the EVOREPRICE 1201-004 project. Israr Ud Din's Ph.D. fellowship was funded by IBGE, The University of Agriculture Peshawar, KPK, Pakistan.