Journal article
Global impact of heparin on gene expression profiles in neural cells infected by Enterovirus 71
HR Pourianfar, E Palombo, L Grollo
Intervirology | KARGER | Published : 2014
DOI: 10.1159/000355872
Abstract
Objectives: Heparan sulphate mimetics, particularly heparin (Hep), have previously been shown to considerably inhibit infection of enterovirus 71 (EV71) in Vero cells. Therefore, in this study, a genome-wide DNA microarray was performed to gain insight into the mechanism(s) of action of Hep against infection of a human neural cell line, SK-N-SH, with a clinical strain of EV71. Methods: This study focused on a selection of EV71-induced genes whose expression profiling was exclusively affected by the antiviral activity of Hep. The selection procedure was performed through a statistical multi-level comparison with the following controls: negative control cells, compound control (cells treated w..
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Funding Acknowledgements
This work was funded by a Grollo Ruzzene Foundation grant awarded to Dr. L. Grollo. The authors wish to thank Dr. Julian Druce of the Victorian Infectious Disease Reference Laboratories (Parkville, Vic., Australia) for providing the clinical strain of EV71. We also thank Drs. Richard Tothil, Tim Holloway, Li Jason, Aga Borcz and other colleagues of the Molecular Genomics Core Facility at the Peter MacCallum Cancer Centre (East Melbourne, Vic., Australia) for processing the microarray hybridization assays and assistance in analysing microarray data.