Spot sputum samples are at least as good as early morning samples for identifying Mycobacterium tuberculosis

ME Murphy; PPJ Phillips; CM Mendel; E Bongard; ALC Bateson; R Hunt; S Murthy; KP Singh; M Brown; AM Crook; AJ Nunn; SK Meredith; M Lipman; TD McHugh; SH Gillespie; A Diacon; M Hanekom; A Venter; R Dawson; K Narunsky; B Mtafya; NE Ntinginya; A Rachow; E Amukoye; B Miheso; M Njoroje; N Sam; D Damas; A Liyoyo; AA Mahayiddin; C Chuchottaworn; J Boonyasopun; B Saipan; S Lakhi; D Chanda; J Mcyeze; A Pym; N Ngcobo; C Louw; H Veldsman; G Amaya-Tapia; TV Aguirre; DK Chauhan; RK Garg; NK Jain; A Aggarwal; M Mishra; S Teotia; S Charalambous; N Hattidge; L Pretorious; N Padayachi; L Mohapi; M Gao; X Li; L Zhang; Q Zhang; S Aggarwal; K Belizaire; M Benhayoun; D Everitt; A Ginsberg; M Laurenzi; B Rawls; C Ridali; M Spigelman; A Uys; C van Niekerk; M Betteridge; S Birkby; H Ciesielczuk; C Cook; E Cunningham; J Huggett; C Ling; P Mee; FMR Perrin; R Shorten; K Smith; V Yorke-Edwards; A Zumla

Journal article

Spot sputum samples are at least as good as early morning samples for identifying Mycobacterium tuberculosis

ME Murphy, PPJ Phillips, CM Mendel, E Bongard, ALC Bateson, R Hunt, S Murthy, KP Singh, M Brown, AM Crook, AJ Nunn, SK Meredith, M Lipman, TD McHugh, SH Gillespie, A Diacon, M Hanekom, A Venter, R Dawson, K Narunsky Show all

BMC Medicine | Published : 2017

DOI: 10.1186/s12916-017-0947-9

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Abstract

Background: The use of early morning sputum samples (EMS) to diagnose tuberculosis (TB) can result in treatment delay given the need for the patient to return to the clinic with the EMS, increasing the chance of patients being lost during their diagnostic workup. However, there is little evidence to support the superiority of EMS over spot sputum samples. In this new analysis of the REMoxTB study, we compare the diagnostic accuracy of EMS with spot samples for identifying Mycobacterium tuberculosis pre- and post-treatment. Methods: Patients who were smear positive at screening were enrolled into the study. Paired sputum samples (one EMS and one spot) were collected at each trial visit pre- a..

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University of Melbourne Researchers

Kasha Singh Author

Related Projects (1)

Subaward: Leadership and Operations Center (LOC), AIDS Clinical Trials Group (ACTG) [UM1AI068636]

Grants

Awarded by National Institute of Allergy and Infectious Diseases

Funding Acknowledgements

Supported by the Global Alliance for TB Drug Development with support from the Bill and Melinda Gates Foundation, the European and Developing Countries Clinical Trials Partnership (Grant IP.2007.32011.011), US Agency for International Development, UK Department for International Development, Directorate General for International Cooperation of the Netherlands, Irish Aid, Australia Department of Foreign Affairs and Trade, National Institutes of Health, AIDS Clinical Trials Group. The study was also supported by grants from the National Institute of Allergy and Infectious Diseases (NIAID) (UM1AI068634, UM1 AI068636, and UM1AI106701) and by NIAID grants to the University of KwaZulu Natal, South Africa, AIDS Clinical Trials Group (ACTG) site 31422 (1U01AI069469); to the Perinatal HIV Research Unit, Chris Hani Baragwanath Hospital, South Africa, ACTG site 12301 (1U01AI069453); and to the Durban International Clinical Trials Unit, South Africa, ACTG site 11201 (1U01AI069426). Bayer Healthcare for donated moxifloxacin and Sanofi donated rifampin.