Swabbing Often Fails to Detect Amphibian Chytridiomycosis under Conditions of Low Infection Load
Jaehyub Shin, Arnaud Bataille, Tiffany A Kosch, Bruce Waldman
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014
The pathogenic chytrid fungus, Batrachochytrium dendrobatidis (denoted Bd), causes large-scale epizootics in naïve amphibian populations. Intervention strategies to rapidly respond to Bd incursions require sensitive and accurate diagnostic methods. Chytridiomycosis usually is assessed by quantitative polymerase chain reaction (qPCR) amplification of amphibian skin swabs. Results based on this method, however, sometimes yield inconsistent results on infection status and inaccurate scores of infection intensity. In Asia and other regions where amphibians typically bear low Bd loads, swab results are least reliable. We developed a Bd-sampling method that collects zoospores released by infected ..View full abstract
Awarded by National Research Foundation of Korea (NRF) - government of the Republic of Korea (MSIP)
The research was supported by the National Research Foundation of Korea (NRF), funded by the government of the Republic of Korea (MSIP) (grants 2010-0002767, 2012R1A1A2044449, and 2012K1A2B1A03000496 to BW), and by Seoul National University (Brain Fusion Program, Brain Korea 21 Program, and New Faculty Resettlement Fund grants to BW). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.