Dynamics of MBD2 deposition across methylated DNA regions during malignant transformation of human mammary epithelial cells
Guillaume Devailly, Melodie Grandin, Laury Perriaud, Pauline Mathot, Jean-Guy Delcros, Yannick Bidet, Anne-Pierre Morel, Jean-Yves Bignon, Alain Puisieux, Patrick Mehlen, Robert Dante
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2015
DNA methylation is thought to induce transcriptional silencing through the combination of two mechanisms: the repulsion of transcriptional activators unable to bind their target sites when methylated, and the recruitment of transcriptional repressors with specific affinity for methylated DNA. The Methyl CpG Binding Domain proteins MeCP2, MBD1 and MBD2 belong to the latter category. Here, we present MBD2 ChIPseq data obtained from the endogenous MBD2 in an isogenic cellular model of oncogenic transformation of human mammary cells. In immortalized (HMEC-hTERT) or transformed (HMLER) cells, MBD2 was found in a large proportion of methylated regions and associated with transcriptional silencing...View full abstract
Ligue Contre le Cancer [to G.D. and P.M.]. LabEx DEVweCAN and Fondation ARC pour la Recherche sur le Cancer [to M.G]. Centre national de la recherche scientifique; University of Lyon; Centre Leon Berard; Ligue Contre le Cancer; Institut National Du Cancer; Agence Nationale de la Recherche; European Research Council; Fondation Bettencourt. Funding for open access charge: Universite Claude Bernard Lyon-1, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex France.