Journal article

Plasmids of Chlamydia psittaci: Cloning and comparison of isolates by Southern hybridisation

P Timms, FW Eaves, AF Hugall, MF Lavin

FEMS Microbiology Letters | Published : 1988


A chlamydial plasmid, 6.2 kb in size, was isolated from an avian strain of Chlamydia psittaci and cloned into the EcoRI site of pUC13. A restriction enzyme cleavage map of the resultant clone, pAP p, was very similar to the published map of the plasmid cloned from the C. psittaci meningopneumonitis strain Cal-10. Southern hybridisation analyses using pAP p as a probe, revealed the presence of plasmids with homologous DNA sequences in avian psittacosis, avian ornithosis, ovine polyarthritis and sporadic bovine encephalomyelitis strains of C. psittaci, as well as the LGV strain of Chlamydia trachomatis. Plasmid was not detected in koala conjunctivitis, ovine abortion or feline conjunctivitis i..

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