Journal article
The nucleoporin Nup2 contains a meiotic-autonomous region that promotes the dynamic chromosome events of meiosis
DB Chu, T Gromova, TAC Newman, SM Burgess
Genetics | GENETICS SOCIETY AMERICA | Published : 2017
Abstract
Meiosis is a specialized cellular program required to create haploid gametes from diploid parent cells. Homologous chromosomes pair, synapse, and recombine in a dynamic environment that accommodates gross chromosome reorganization and significant chromosome motion, which are critical for normal chromosome segregation. In Saccharomyces cerevisiae, Ndj1 is a meiotic telomere-associated protein required for physically attaching telomeres to proteins embedded in the nuclear envelope. In this study, we identified additional proteins that act at the nuclear periphery from meiotic cell extracts, including Nup2, a nonessential nucleoporin with a known role in tethering interstitial chromosomal loci ..
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Awarded by National Institutes of Health
Funding Acknowledgements
We would like to thank Dan Starr, JoAnne Engebrecht, Anne Britt, James McGehee, and An Nguyen for their insightful review of this manuscript, and Soni Lacefield for discussion. We thank Yana Blokhina for assistance with imaging and Kelsey Walters for assistance in strain construction. We thank the Kaback laboratory for the ZIP1-GFP plasmid, Neil Hunter for strains, and Akira Shinohara for sharing detailed protocols. The authors have declared that no conflicting interests exist. This work was funded by National Institutes of Health (NIH) R01 GM075119 award to S.M.B. and pilot funds from the University of California Davis Proteomics Core.