Journal article

Interactions of core cross-linked poly(2-oxazoline) and poly(2-oxazine) micelles with immune cells in human blood

Meike N Leiske, May Lai, Thakshila Amarasena, Thomas P Davis, Kristofer J Thurecht, Stephen J Kent, Kristian Kempe

Biomaterials | Elsevier | Published : 2021

Abstract

Water-soluble poly(cyclic imino ether)s (PCIEs) have emerged as promising biocompatible polymers for nanomedicine applications in recent years. Despite their generally accepted stealth properties, there has been no comprehensive evaluation of their interactions with primary immune cells in human blood. Here we present a library of core cross-linked micelles (CCMs) containing various PCIE shells. Well-defined high molar mass CCMs (Mn > 175 kDa, Р< 1.2) of similar diameter (~20 nm) were synthesised using a cationic ring-opening polymerisation (CROP) - surfactant-free reversible addition-fragmentation chain-transfer (RAFT) emulsion polymerisation strategy. The stealth properties of the differe..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Awarded by NHMRC


Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science Technology


Funding Acknowledgements

K.K. gratefully acknowledges the award of an ARC Future Fellowship (FT190100572) from the Australian Research Council. M.N.L. acknowledges the Alexander von Humboldt Foundation for financial support and K.J.T. and S.J.K. acknowledge the NHMRC for fellowship support (APP1148582, APP1136322). This work was carried out -in part-within the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science & Technology (Project No. CE140100036). The authors acknowledge the use of instruments and scientific and technical assistance at the Monash Centre for Electron Microscopy (MCEM), a Node of Microscopy Australia. The authors acknowledge the use of the facilities and the assistance of Dr Timothy Williams, Dr Yu (Emily) Chen and Dr Russel King at MCEM.