Journal article

Longitudinal hippocampal volumetric changes in mice following brain infarction

Vanessa H Brait, David K Wright, Mohsen Nategh, Alexander Oman, Warda T Syeda, Charlotte M Ermine, Katrina R O'Brien, Emilio Werden, Leonid Churilov, Leigh A Johnston, Lachlan H Thompson, Jess Nithianantharajah, Katherine A Jackman, Amy Brodtmann

SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021

Abstract

Hippocampal atrophy is increasingly described in many neurodegenerative syndromes in humans, including stroke and vascular cognitive impairment. However, the progression of brain volume changes after stroke in rodent models is poorly characterized. We aimed to monitor hippocampal atrophy occurring in mice up to 48-weeks post-stroke. Male C57BL/6J mice were subjected to an intraluminal filament-induced middle cerebral artery occlusion (MCAO). At baseline, 3-days, and 1-, 4-, 12-, 24-, 36- and 48-weeks post-surgery, we measured sensorimotor behavior and hippocampal volumes from T2-weighted MRI scans. Hippocampal volume-both ipsilateral and contralateral-increased over the life-span of sham-ope..

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Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by Australian Research Council Future Fellowship


Awarded by NHMRC Career Development Fellowship


Awarded by Heart Foundation Future Leader Fellowship


Funding Acknowledgements

These studies were supported by a Dementia Research Team Grant from the National Health and Medical Research Council of Australia (NHMRC; GNT1094974). J.N. was supported by an Australian Research Council Future Fellowship (FT140101327). A.B. was supported by an NHMRC Career Development Fellowship (GNT1045617) and a Heart Foundation Future Leader Fellowship (HFFLF100784). The authors acknowledge the facilities, and the scientific and technical assistance of the National Imaging Facility at the Florey Node.