Journal article

Dopamine Transporter Is a Master Regulator of Dopaminergic Neural Network Connectivity

Douglas R Miller, Dylan T Guenther, Andrew P Maurer, Carissa A Hansen, Andrew Zalesky, Habibeh Khoshbouei

JOURNAL OF NEUROSCIENCE | SOC NEUROSCIENCE | Published : 2021

Abstract

Dopaminergic neurons of the substantia nigra pars compacta (SNC) and ventral tegmental area (VTA) exhibit spontaneous firing activity. The dopaminergic neurons in these regions have been shown to exhibit differential sensitivity to neuronal loss and psychostimulants targeting dopamine transporter. However, it remains unclear whether these regional differences scale beyond individual neuronal activity to regional neuronal networks. Here, we used live-cell calcium imaging to show that network connectivity greatly differs between SNC and VTA regions with higher incidence of hub-like neurons in the VTA. Specifically, the frequency of hub-like neurons was significantly lower in SNC than in the ad..

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University of Melbourne Researchers

Grants

Awarded by National Institute of Neurological Disorders and Stroke


Funding Acknowledgements

D.R.M. was supported by the National Institute of Neurological Disorders and Stroke Grant T32NS082128. H.K. was supported by National Institute on Drug Abuse and National Institute of Neurological Disorders and Stroke Grants R01-DA026947 and R01NS071122. We thank Dr. Jeff Beeler for his insightful comments that improved the quality of the manuscript.