Journal article

Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain

Michal E Mor, Alexandra Harvey, Mary Familari, Mitchell St Clair-Glover, Serena Viventi, Robb U de Iongh, Fergus J Cameron, Mirella Dottori

BRAIN RESEARCH BULLETIN | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021

Abstract

Cortical neurospheres (NSPs) derived from human pluripotent stem cells (hPSC), have proven to be a successful platform to investigate human brain development and neuro-related diseases. Currently, many of the standard hPSC neural differentiation media, use concentrations of glucose (approximately 17.5-25 mM) and insulin (approximately 3.2 μM) that are much greater than the physiological concentrations found in the human brain. These culture conditions make it difficult to analyse perturbations of glucose or insulin on neuronal development and differentiation. We established a new hPSC neural differentiation medium that incorporated physiological brain concentrations of glucose (2.5 mM) and s..

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Grants

Funding Acknowledgements

M. E. Mor was supported by Melbourne Research Scholarship (MRS) . This work was supported by the Murdoch Children's Research Institute, The University of Melbourne, the Australian Research Council, and Stem Cells Australia. We thank A/Prof Kaylene J Simpson, Arthi M Macpherson and Twishi Gulati from the Victorian Centre for Functional Genomics and the ACRF Translational RPPA platform at the Peter MacCallum Cancer, for generating the RPPA data.