Journal article

A Putative Lipid-Associating Motif in the West Nile Virus NS4A Protein Is Required for Efficient Virus Replication

Andrea Mikulasova, Leah K Gillespie, Rebecca L Ambrose, Turgut E Aktepe, Alice M Trenerry, Susann Liebscher, Jason M Mackenzie

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | FRONTIERS MEDIA SA | Published : 2021

Abstract

Flavivirus replication is intimately associated with re-organized cellular membranes. These virus-induced changes in membrane architecture form three distinct membranous "organelles" that have specific functions during the flavivirus life cycle. One of these structures is the replication complex in which the flaviviral RNA is replicated to produce progeny genomes. We have previously observed that this process is strictly dependent on cellular cholesterol. In this study we have identified a putative cholesterol recognition/interaction amino acid consensus (CRAC) motif within the West Nile virus strain Kunjin virus (WNVKUN) NS4A protein. Site-directed mutagenesis of this motif within a WNVKUN ..

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