Journal article

T cell receptor beta locus sequencing early post-allogeneic stem cell transplant identifies patients at risk of initial and recurrent cytomegalovirus infection

James A Kuzich, Yamuna Kankanige, Jerick Guinto, Georgina Ryland, Michelle McBean, Eric Wong, Rachel Koldej, Jenny Collins, David Westerman, David Ritchie, Piers Blombery

BONE MARROW TRANSPLANTATION | SPRINGERNATURE | Published : 2021

Abstract

Identification of patients at risk of initial & recurrent cytomegalovirus (CMV) reactivation following allogeneic stem cell transplant (alloSCT) may help guide prophylactic strategies. T-cell receptor beta (TRB) deep sequencing was used to identify and enumerate the T-cell repertoire harbouring TRB sequences with annotated specificity to CMV (pubCMVrep), as well as the overall T-cell receptor (TCR) repertoire diversity at day +30 & day +60 post-alloSCT for 65 patients. T-cells harbouring TRB sequences with annotated specificity for CMV were identifiable in all patients. 56% of patients required CMV treatment and 23% of the cohort developed recurrent CMV. PubCMVrep size at day +30 was not ass..

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Grants

Funding Acknowledgements

This work was supported by grants from the Wilson Centre for Lymphoma Genomics and the Snowdome Foundation.