Journal article

Flagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm

Ashwin Nandagiri, Avinash Satish Gaikwad, David L Potter, Reza Nosrati, Julio Soria, Moira K O'Bryan, Sameer Jadhav, Ranganathan Prabhakar

ELIFE | ELIFE SCIENCES PUBLICATIONS LTD | Published : 2021

Abstract

We demonstrate a technique for investigating the energetics of flagella or cilia. We record the planar beating of tethered mouse sperm at high resolution. Beating waveforms are reconstructed using proper orthogonal decomposition of the centerline tangent-angle profiles. Energy conservation is employed to obtain the mechanical power exerted by the dynein motors from the observed kinematics. A large proportion of the mechanical power exerted by the dynein motors is dissipated internally by the motors themselves. There could also be significant dissipation within the passive structures of the flagellum. The total internal dissipation is considerably greater than the hydrodynamic dissipation in ..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Awarded by Department of Biotechnology, Ministry of Science and Technology


Funding Acknowledgements

Australian Research Council DP190100343 Reza Nosrati Ranganathan PrabhakarAustralian Research Council DP200100659 Moira K O'BryanDepartment of Biotechnology, Ministry of Science and Technology BT/PR13442/MED/32/440/2015 Sameer JadhavThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.