New Insights Into Sperm Ultrastructure Through Enhanced Scanning Electron Microscopy
Denis Korneev, D Jo Merriner, Gediminas Gervinskas, Alex de Marco, Moira K O'Bryan
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | FRONTIERS MEDIA SA | Published : 2021
The analysis of spermatozoa morphology is fundamental to understand male fertility and the etiology of infertility. Traditionally scanning electron microscopy (SEM) has been used to define surface topology. Recently, however, it has become a critical tool for three-dimensional analysis of internal cellular ultrastructure. Modern SEM provides nanometer-scale resolution, but the meaningfulness of such information is proportional to the quality of the sample preservation. In this study, we demonstrate that sperm quickly and robustly adhere to gold-coated surfaces. Leveraging this property, we developed three step-by-step protocols fulfilling different needs for sperm imaging: chemically fixed m..View full abstract
Awarded by Australian Research Council
Awarded by Australian Research Council Grant(s) FEI Helios Cryo FIBSEM - ARC LIEF Fund
This research was funded by an Australian Research Council Discovery Project Grant (DP180100533) to MO'B. This research used equipment funded by Australian Research Council Grant(s) FEI Helios Cryo FIBSEM -ARC LIEF Funding (LE150100132).