Journal article

Influence of protein ion charge state on 213 nm top-down UVPD

Simon Becher, Huixin Wang, Michael G Leeming, William A Donald, Sven Heiles

ANALYST | ROYAL SOC CHEMISTRY | Published : 2021

Abstract

Ultraviolet photodissociation (UVPD) is a powerful and rapidly developing method in top-down proteomics. Sequence coverages can exceed those obtained with collision- and electron-induced fragmentation methods. Because of the recent interest in UVPD, factors that influence protein fragmentation and sequence coverage are actively debated in the literature. Here, we performed top-down 213 nm UVPD experiments on a 7 T Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) for the model proteins ubiquitin, myoglobin and cytochrome c that were electrosprayed from native, denaturing and supercharging solutions in order to investigate the effect of protein charge states on UVPD frag..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Funding Acknowledgements

S.H. acknowledges the Fonds der chemischen Industrie for financial support through a Liebig fellowship. The authors thank Alexander S. Berninger for initial support during this project and Marion Girod for providing their UVPD data. W.A.D. thanks the Australian Research Council for funding (FT200100798).