Influence of protein ion charge state on 213 nm top-down UVPD
Simon Becher, Huixin Wang, Michael G Leeming, William A Donald, Sven Heiles
ANALYST | ROYAL SOC CHEMISTRY | Published : 2021
Ultraviolet photodissociation (UVPD) is a powerful and rapidly developing method in top-down proteomics. Sequence coverages can exceed those obtained with collision- and electron-induced fragmentation methods. Because of the recent interest in UVPD, factors that influence protein fragmentation and sequence coverage are actively debated in the literature. Here, we performed top-down 213 nm UVPD experiments on a 7 T Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) for the model proteins ubiquitin, myoglobin and cytochrome c that were electrosprayed from native, denaturing and supercharging solutions in order to investigate the effect of protein charge states on UVPD frag..View full abstract
Awarded by Australian Research Council
S.H. acknowledges the Fonds der chemischen Industrie for financial support through a Liebig fellowship. The authors thank Alexander S. Berninger for initial support during this project and Marion Girod for providing their UVPD data. W.A.D. thanks the Australian Research Council for funding (FT200100798).