Journal article

Infrared Based Saliva Screening Test for COVID-19

Bayden R Wood, Kamila Kochan, Diana E Bedolla, Natalia Salazar-Quiroz, Samantha L Grimley, David Perez-Guaita, Matthew J Baker, Jitraporn Vongsvivut, Mark J Tobin, Keith R Bambery, Dale Christensen, Shivani Pasricha, Anthony K Eden, Aaron Mclean, Supti Roy, Jason A Roberts, Julian Druce, Deborah A Williamson, Julie McAuley, Mike Catton Show all

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | WILEY-V C H VERLAG GMBH | Published : 2021

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sen..

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Grants

Awarded by Australian Government


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by Australian Research Council


Awarded by Ramln y Cajal (RYC) Contracts Aids


Funding Acknowledgements

Mr. Finlay Shanks for instrumental support. Dr Emanuele Pedersoli for his assistance in graphic scripting. Funding: Partof this research was undertaken on the IRM beamline at Australian Synchrotron (Victoria, Australia), part of the Australian Nuclear Science and Technology Organisation (ANSTO). We acknowledge the support of the beamtime (Proposal ID. 16460 and 16476) provided by ANSTO, funded by the Australian Government. This work was in-part funded by Monash Green Chemical Futures Special Project fund. Part of this work was funded by the A2 milk foundation. Instruments were loaned and modified for transflection infrared slides by Dr Jerry Sellors and Dr Robert Packer courtesy of PerkinElmerU. N.S.Q, S.G. and J.M. were supported by funds from the Jack Ma Foundation through a grant to D.P. and D.I.G. D.P. and D.I.G. were supported by the National Health and Medical Research Council of Australia (NHMRC), including a Senior Principal Research Fellowship (1117766) and the Australian Research Council CE140100011 for D.I.G. D.P.-G. acknowledges the financial support from the 2019 Ramln y Cajal (RYC) Contracts Aids (RYC2019026556-I).