Journal article

Viral-mediated transduction of auditory neurons with opsins for optical and hybrid activation

Rachael T Richardson, Alex C Thompson, Andrew K Wise, Elise A Ajay, Niliksha Gunewardene, Stephen J O'Leary, Paul R Stoddart, James B Fallon

SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021

Abstract

Optical stimulation is a paradigm-shifting approach to modulating neural activity that has the potential to overcome the issue of current spread that occurs with electrical stimulation by providing focused stimuli. But optical stimulation either requires high power infrared light or genetic modification of neurons to make them responsive to lower power visible light. This work examines optical activation of auditory neurons following optogenetic modification via AAV injection in two species (mouse and guinea pig). An Anc80 viral vector was used to express the channelrhodopsin variant ChR2-H134R fused to a fluorescent reporter gene under the control of the human synapsin-1 promoter. The AAV w..

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Grants

Awarded by Royal National Institute for Deaf People (RNID) Project


Funding Acknowledgements

pAAV-hSyn-hChR2(H134R)-EYFP was a gift from Karl Deisseroth (Addgene plasmid #26973). The authors acknowledge the technical support provided by Trung Nguyen, Brianna Flynn, Ella Trang, Caitlin Singleton, Donna Tuari, Sayward Barone, Peta Grigsby, Dilys Lam and Matteus Harrington. This work was supported with funding from the Garnett Passe and Rodney Williams Memorial Foundation and Royal National Institute for Deaf People (RNID) Project Grant G89. The Bionics Institute acknowledges the support it receives from the Victorian Government through its Operational Infrastructure Support Program.