Application of a targeted-enrichment methodology for full-genome sequencing of Dengue 1-4, Chikungunya and Zika viruses directly from patient samples
Uma Sangumathi Kamaraj, Jun Hao Tan, Ong Xin Mei, Louise Pan, Tanu Chawla, Anna Uehara, Lin-Fa Wang, Eng Eong Ooi, Duane J Gubler, Hasitha Tissera, Lee Ching Ng, Annelies Wilder-Smith, Paola Florez de Sessions, Timothy Barkham, Danielle E Anderson, October Michael Sessions
PLOS Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2019
The frequency of epidemics caused by Dengue viruses 1-4, Zika virus and Chikungunya viruses have been on an upward trend in recent years driven primarily by uncontrolled urbanization, mobility of human populations and geographical spread of their shared vectors, Aedes aegypti and Aedes albopictus. Infections by these viruses present with similar clinical manifestations making them challenging to diagnose; this is especially difficult in regions of the world hyperendemic for these viruses. In this study, we present a targeted-enrichment methodology to simultaneously sequence the complete viral genomes for each of these viruses directly from clinical samples. Additionally, we have also develop..View full abstract
Awarded by Singapore National Medical Research Council
Awarded by European Union
We would also like to thank the Singapore National Medical Research Council (http://www.nmrc.gov.sg) for supporting this study through grants to OMS (NMRC/BNIG/2014/2014) and EEO (CIRG13may040) and the European Union (https://cordis.europa.eu/result/rcn/189834 en.html) for the grant to HT and AWS ("DengueTools" - 282589). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.