Journal article

Comparative Loss-of-Function Screens Reveal ABCE1 as an Essential Cellular Host Factor for Efficient Translation of Paramyxoviridae and Pneumoviridae

Danielle E Anderson, Kristin Pfeffermann, So Young Kim, Bevan Sawatsky, James Pearson, Mikhail Kovtun, David L Corcoran, Yvonne Krebs, Kristmundur Sigmundsson, Sharon F Jamison, Zhen Zhen Joanna Yeo, Linda J Rennick, Lin-Fa Wang, Pierre J Talbot, W Paul Duprex, Mariano A Garcia-Blanco, Veronika von Messling

mBio | AMER SOC MICROBIOLOGY | Published : 2019

Abstract

Paramyxoviruses and pneumoviruses have similar life cycles and share the respiratory tract as a point of entry. In comparative genome-scale siRNA screens with wild-type-derived measles, mumps, and respiratory syncytial viruses in A549 cells, a human lung adenocarcinoma cell line, we identified vesicular transport, RNA processing pathways, and translation as the top pathways required by all three viruses. As the top hit in the translation pathway, ABCE1, a member of the ATP-binding cassette transporters, was chosen for further study. We found that ABCE1 supports replication of all three viruses, confirming its importance for viruses of both families. More detailed characterization revealed th..

View full abstract

Grants

Awarded by Canadian Institutes of Health Research


Awarded by Canada Foundation for Innovation


Awarded by DZIF TTU Emerging Infections of the German Center for Infection Research


Awarded by USA NIH



Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by grants CIHR MOP-66989 of the Canadian Institutes of Health Research, CFI 9488 Canada Foundation for Innovation, DZIF TTU Emerging Infections SFB1021 of the German Center for Infection Research, and intramural funding of the German Ministry of Health to V.V.M.; NMRC/BNIG/2030/2015 (Singapore) to D.E.A.; and USA NIH R01-AI089526 and R01-AI101431 to M.A.G.-B. and R01-AI099100 to W.P.D.