Journal article

Effects of a major deletion in the SARS-CoV-2 genome on the severity of infection and the inflammatory response: an observational cohort study

Barnaby E Young, Siew-Wai Fong, Yi-Hao Chan, Tze-Minn Mak, Li Wei Ang, Danielle E Anderson, Cheryl Yi-Pin Lee, Siti Naqiah Amrun, Bernett Lee, Yun Shan Goh, Yvonne CF Su, Wycliffe E Wei, Shirin Kalimuddin, Louis Yi Ann Chai, Surinder Pada, Seow Yen Tan, Louisa Sun, Purnima Parthasarathy, Yuan Yi Constance Chen, Timothy Barkham Show all

The Lancet | ELSEVIER SCIENCE INC | Published : 2020

Abstract

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with a 382-nucleotide deletion (∆382) in the open reading frame 8 (ORF8) region of the genome have been detected in Singapore and other countries. We investigated the effect of this deletion on the clinical features of infection. METHODS: We retrospectively identified patients who had been screened for the ∆382 variant and recruited to the PROTECT study-a prospective observational cohort study conducted at seven public hospitals in Singapore. We collected clinical, laboratory, and radiological data from patients' electronic medical records and serial blood and respiratory samples taken during hospitalisation an..

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Grants

Awarded by Singapore National Medical Research Council's COVID-19 Research Fund


Awarded by Biomedical Research Council COVID-19 fund


Awarded by A*ccelerate GAP from the Agency of Science, Technology, and Research


Awarded by National Institute of Allergy and Infectious Diseases (National Institutes of Health, US Department of Health and Human Services)


Funding Acknowledgements

National Medical Research Council Singapore.This study was funded by grants from the Singapore National Medical Research Council's COVID-19 Research Fund (grant numbers COVID19RF-001, COVID19RF-004, and COVID19RF-007), the Biomedical Research Council COVID-19 fund (H20/04/g1/006), and the A*ccelerate GAP-funded project (ACCL/19-GAP064-R20H-H) from the Agency of Science, Technology, and Research. YCFS and GJDS are supported by contract HHSN272201400006C from the National Institute of Allergy and Infectious Diseases (National Institutes of Health, US Department of Health and Human Services). We thank all clinical and nursing staff who provided care for the patients; staff at the Communicable Diseases Division of the Ministry of Health Singapore who contributed to the outbreak response and contact tracing; staff at the National Public Health and Epidemiology Unit of the National Centre for Infectious Diseases who assisted with data analysis; staff in the Singapore Infectious Disease Clinical Research Network and Infectious Disease Research and Training Office of the National Centre for Infectious Diseases for coordinating patient recruitment.