Elements in the Canine Distemper Virus M 3 ' UTR Contribute to Control of Replication Efficiency and Virulence
Danielle E Anderson, Alexandre Castan, Martin Bisaillon, Veronika von Messling
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012
Canine distemper virus (CDV) is a negative-sense, single-stranded RNA virus within the genus Morbillivirus and the family Paramyxoviridae. The Morbillivirus genome is composed of six transcriptional units that are separated by untranslated regions (UTRs), which are relatively uniform in length, with the exception of the UTR between the matrix (M) and fusion (F) genes. This UTR is at least three times longer and in the case of CDV also highly variable. Exchange of the M-F region between different CDV strains did not affect virulence or disease phenotype, demonstrating that this region is functionally interchangeable. Viruses carrying the deletions in the M 3' UTR replicated more efficiently, ..View full abstract
Awarded by Canadian Institutes of Health Research (CIHR)
Awarded by Canadian Foundation for Innovation (CFI)
This work was supported by grants from the Canadian Institutes of Health Research (CIHR; MOP-66989) and the Canadian Foundation for Innovation (CFI; 9488) to V.v.M., a Fonds de la Recherche en Sante Quebec (FRSQ) fellowship to D.E.A, and a Fondation Armand-Frappier Scholarship to A.C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.