Journal article
Detection of recombinant rousettus bat coronavirus GCCDC1 in lesser dawn bats (eonycteris spelaea) in Singapore
AC Paskey, JHJ Ng, GK Rice, WN Chia, CW Philipson, RJH Foo, RZ Cer, KA Long, MR Lueder, XF Lim, KG Frey, T Hamilton, DE Anderson, ED Laing, IH Mendenhall, GJ Smith, LF Wang, KA Bishop-Lilly
Viruses | MDPI | Published : 2020
DOI: 10.3390/v12050539
Abstract
Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. RoBat-CoV GCCDC1 Singapore shared 96.52% similarity with RoBat-CoV GCCDC1 356 (NC-030886) at the nucleotide level, and had a high prevalence in the captive bat colony. It was detected at five out of six sampling time points across the course of 18 months. A parti..
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Awarded by Office of Naval Research
Funding Acknowledgements
This work was funded by U.S. Navy, Office of Naval Research, In-House Laboratory Independent Research (ILIR) Program and WUN A1417. The work by the Singapore team was funded by the Singapore National Research Foundation grants (NRF2012NRF-CRP001-056 and NRF2016NRF-NSFC002-013). The funding bodies had no role in the design of this study, nor in the collection, analysis, or interpretation of data, nor in the writing of this manuscript.