Function of the Small Hydrophobic Protein of J Paramyxovirus
Zhuo Li, Jie Xu, Jui Patel, Sandra Fuentes, Yuan Lin, Danielle Anderson, Kaori Sakamoto, Lin-Fa Wang, Biao He
Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2011
At 18,954 nucleotides, the J paramyxovirus (JPV) genome is one of the largest in the family Paramyxoviridae, consisting of eight genes in the order 3'-N-P/V/C-M-F-SH-TM-G-L-5'. To study the function of novel paramyxovirus genes in JPV, a plasmid containing a full-length cDNA clone of the genome of JPV was constructed. In this study, the function of the small hydrophobic (SH) protein of JPV was examined by generating a recombinant JPV lacking the coding sequence of the SH protein (rJPVΔSH). rJPVΔSH was viable and had no growth defect in tissue culture cells. However, more tumor necrosis factor alpha (TNF-α) was produced during rJPVΔSH infection, suggesting that SH plays a role in inhibiting T..View full abstract
Awarded by Georgia Research Alliance
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
The work was supported by grants from the National Institute of Allergy and Infectious Diseases and Georgia Research Alliance to B. H. (grant numbers AI070847 and K02 AI65795).