Journal article

Function of the Small Hydrophobic Protein of J Paramyxovirus

Zhuo Li, Jie Xu, Jui Patel, Sandra Fuentes, Yuan Lin, Danielle Anderson, Kaori Sakamoto, Lin-Fa Wang, Biao He

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2011

Abstract

At 18,954 nucleotides, the J paramyxovirus (JPV) genome is one of the largest in the family Paramyxoviridae, consisting of eight genes in the order 3'-N-P/V/C-M-F-SH-TM-G-L-5'. To study the function of novel paramyxovirus genes in JPV, a plasmid containing a full-length cDNA clone of the genome of JPV was constructed. In this study, the function of the small hydrophobic (SH) protein of JPV was examined by generating a recombinant JPV lacking the coding sequence of the SH protein (rJPVΔSH). rJPVΔSH was viable and had no growth defect in tissue culture cells. However, more tumor necrosis factor alpha (TNF-α) was produced during rJPVΔSH infection, suggesting that SH plays a role in inhibiting T..

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