Detection and characterization of a novel bat-borne coronavirus in Singapore using multiple molecular approaches
Xiao Fang Lim, Chengfa Benjamin Lee, Sarah Marie Pascoe, Choon Beng How, Sharon Chan, Jun Hao Tan, Xinglou Yang, Peng Zhou, Zhengli Shi, October M Sessions, Lin-Fa Wang, Lee Ching Ng, Danielle E Anderson, Grace Yap
Journal of General Virology | MICROBIOLOGY SOC | Published : 2019
Bats are important reservoirs and vectors in the transmission of emerging infectious diseases. Many highly pathogenic viruses such as SARS-CoV and rabies-related lyssaviruses have crossed species barriers to infect humans and other animals. In this study we monitored the major roost sites of bats in Singapore, and performed surveillance for zoonotic pathogens in these bats. Screening of guano samples collected during the survey uncovered a bat coronavirus (Betacoronavirus) in Cynopterus brachyotis, commonly known as the lesser dog-faced fruit bat. Using a capture-enrichment sequencing platform, the full-length genome of the bat CoV was sequenced and found to be closely related to the bat cor..View full abstract
Awarded by Ministry of Environment and Water Resources, Ministry of Finance
Awarded by Defence Innovative Research Programme
Awarded by National Research Foundation, Communicable Diseases-Public Health Research Grant
This work was jointly supported by NRF2016NRF-NSFC002-013 (L.-F. W., P. Z., Z. S. and D. E. A.). The project was funded in part by the Ministry of Environment and Water Resources, Ministry of Finance, Communicable Diseases-Public Health Research Grant CDPHRG/0006/2014 (L. -F. W. and D. E. A.), Defence Innovative Research Programme Grant MINDEF-NUS-DIRP/2015/05 (L. -F. W. and D. E. A.) and National Research Foundation grant NRF-CRP10-2012-05 (L. -F. W.).