Journal article

Serological evidence and experimental infection of cynomolgus macaques with pteropine orthorevirus reveal monkeys as potential hosts for transmission to humans

Chee Wah Tan, Kevin Wittwer, Xiao Fang Lim, Anna Uehara, Shailendra Mani, Lin-Fa Wang, Danielle E Anderson

Emerging Microbes & Infections | TAYLOR & FRANCIS LTD | Published : 2019

Abstract

Pteropine orthoreoviruses (PRV) are emerging bat-borne viruses with proven zoonotic transmission. We recently demonstrated human exposure to PRV in Singapore, which together with previous reports from Malaysia and Vietnam suggest that human infection of PRV may occur periodically in the region. This raises the question whether bats are the only sources of human infection. In this study, we screened 517 cynomolgus macaques caught in Singapore for evidence of exposure to PRV3M (also known as Melaka virus), which was first isolated from human patients in Melaka, Malaysia. We found that 67 serum samples were PRV3M positive by ELISA and 34 were also positive by virus neutralization assay. To inve..

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Grants

Awarded by Communicable Diseases-Public Health Research Grant


Awarded by Ministry of Defence under the Defence Innovative Research Programme


Awarded by National Research Foundation Singapore


Funding Acknowledgements

The project was funded in part by a Communicable Diseases-Public Health Research Grant CDPHRG/0006/2014 (L-FW and DEA), Ministry of Defence under the Defence Innovative Research Programme Grant 9016102060 (L-FW and DEA) and National Research Foundation Singapore grant NRF-CRP10-2012-05 (L-FW), NRF2016NRF-NSFC002-013 (L-FW and DEA) and NRF2018NRF-NSFC003SB-002 (L-FW and DEA).