Journal article

Live cell dynamics of the NF-Y transcription factor

David G Priest, Andrea Bernardini, Jieqiong Lou, Roberto Mantovani, Elizabeth Hinde

SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021

Abstract

Transcription factors (TFs) are core players in the control of gene expression, evolutionarily selected to recognise a subset of specific DNA sequences and nucleate the recruitment of the transcriptional machinery. How TFs assemble and move in the nucleus to locate and bind their DNA targets and cause a transcriptional response, remains mostly unclear. NF-Y is a highly conserved, heterotrimeric TF with important roles in both housekeeping and lineage-specific gene expression, functioning as a promoter organiser. Despite a large number of biochemical, structural and genomic studies of NF-Y, there is a lack of experiments in single living cells; therefore, basic assumptions of NF-Y biology rem..

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Grants

Awarded by Italian Association for Cancer Research Investigator Grant


Awarded by Australian Research Council (ARC)


Awarded by National Health and Medical Research Council (NHMRC) Career Development Fellowship


Funding Acknowledgements

We acknowledge instrument training and support provided by the Biological Optical Microscopy Platform (BOMP), the University of Melbourne. D.P. acknowledges support from a University of Melbourne Early Career Researcher (ECR) grant (2019). This work was also supported by an Italian Association for Cancer Research Investigator Grant IG 2016/IG-19050 (to R.M.), an Australian Research Council (ARC) discovery project (DP180101387) (to D.P and J.L), a National Health and Medical Research Council (NHMRC) Career Development Fellowship (APP1124762) (to E.H.) and the Jacob Haimson Beverly Mecklenburg Lectureship (to E.H).