Journal article

A multipronged next-generation sequencing metabarcoding approach unearths hyper-diverse and abundant dog pathogen communities in Cambodia

Lucas G Huggins, Vito Colella, Anson V Koehler, Bettina Schunack, Rebecca J Traub

Transboundary and Emerging Diseases | Wiley | Published : 2021

Abstract

Recent surveys in Southeast Asia, including Cambodia, have identified canine vector-borne pathogens (VBPs), including those with zoonotic potential, as highly prevalent. The lack of veterinary care alongside the close association semi-domesticated dogs have with humans in the region exacerbates these zoonotic risks. Nonetheless, the number of studies investigating such pathogens and the threats they pose to dog and human health is limited. Here, we utilise a next-generation sequencing (NGS) based metabarcoding protocol to conduct an assumption-free characterisation of the bacterial, apicomplexan and kinetoplastid blood-borne pathogens of free-roaming dogs from across Cambodia. From 467 dogs ..

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