OBF1 and Oct factors control the germinal center transcriptional program
Shuang Song, Chun Cao, Mohamed-Amin Choukrallah, Fengyuan Tang, Gerhard Christofori, Hubertus Kohler, Fabian Wu, Barna D Fodor, Mathias Frederiksen, Simon N Willis, Jacob T Jackson, Stephen L Nutt, Stefan Dirnhofer, Michael B Stadler, Patrick Matthias
BLOOD | AMER SOC HEMATOLOGY | Published : 2021
OBF1 is a specific coactivator of the POU family transcription factors OCT1 and OCT2. OBF1 and OCT2 are B cell-specific and indispensable for germinal center (GC) formation, but their mechanism of action is unclear. Here, we show by chromatin immunoprecipitation-sequencing that OBF1 extensively colocalizes with OCT1 and OCT2. We found that these factors also often colocalize with transcription factors of the ETS family. Furthermore, we showed that OBF1, OCT2, and OCT1 bind widely to the promoters or enhancers of genes involved in GC formation in mouse and human GC B cells. Short hairpin RNA knockdown experiments demonstrated that OCT1, OCT2, and OBF1 regulate each other and are essential for..View full abstract
This work was supported by the Novartis Research Foundation.