Optimization of behavioral testing in a long-term rat model of hypoxic ischemic brain injury.
Tayla R Penny, Yen Pham, Amy E Sutherland, Madeleine J Smith, Joohyung Lee, Graham Jenkin, Michael C Fahey, Suzanne L Miller, Courtney A McDonald
Behav Brain Res | Published : 2021
BACKGROUND: Hypoxic ischemic (HI) brain injury is a significant cause of childhood neurological deficits. Preclinical rodent models are often used to study these deficits; however, no preclinical study has determined which behavioral tests are most appropriate for long-term follow up after neonatal HI. METHODS: HI brain injury was induced in postnatal day (PND) 10 rat pups using the Rice-Vannucci method of unilateral carotid artery ligation. Rats underwent long-term behavioral testing to assess motor and cognitive outcomes between PND11-50. Behavioral scores were transformed into Z-scores and combined to create composite behavioral scores. RESULTS: HI rats showed a significant deficit in thr..View full abstract