Classification of molecular subtypes of high-grade serous ovarian cancer by MALDI-Imaging.
Wanja Nikolai Kassuhn, Oliver Klein, Silvia Darb-Esfahani, Hedwig Lammert, Sylwia Handzik, Eliane T Taube, Wolfgang D Schmitt, Oliver Dorigo, David Horst, Carlotta Keunecke, Felix Dreher, Joshy George, David Bowtell, Michael Hummel, Jalid Sehouli, Nils Blüthgen, Hagen Kulbe, Elena Ioana Braicu
Journal of Clinical Oncology | American Society of Clinical Oncology (ASCO) | Published : 2021
e17544 Background: High-grade serous ovarian cancer (HGSOC) can be separated by gene expression profiling into four molecular subtypes with clear correlation of the clinical outcome. However, these gene signatures have not been implemented in clinical practice to stratify patients for targeted therapy. This is mainly due to a lack of easy, cost-effective and reproducible methods, as well as the high heterogeneity of HGSOC. Hence, we aimed to examine the potential of unsupervised matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to stratify patients, which might benefit from targeted therapeutic strategies. Methods: Molecular subtyping of paraffin-embedded tis..View full abstract