Journal article

TDM1 Regulation Determines the Number of Meiotic Divisions

Marta Cifuentes, Sylvie Jolivet, Laurence Cromer, Hirofumi Harashima, Petra Bulankova, Charlotte Renne, Wayne Crismani, Yuko Nomura, Hirofumi Nakagami, Keiko Sugimoto, Arp Schnittger, Karel Riha, Raphael Mercier

PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2016

Abstract

Cell cycle control must be modified at meiosis to allow two divisions to follow a single round of DNA replication, resulting in ploidy reduction. The mechanisms that ensure meiosis termination at the end of the second and not at the end of first division are poorly understood. We show here that Arabidopsis thaliana TDM1, which has been previously shown to be essential for meiotic termination, interacts directly with the Anaphase-Promoting Complex. Further, mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division, and the production of diploid spores and gametes. The CDKA;1..

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Grants

Awarded by Czech Science Foundation


Awarded by Japan Society for the Promotion of Science (JSPS)


Awarded by European Research Council


Awarded by Labex Saclay Plant Sciences-SPS


Awarded by Grants-in-Aid for Scientific Research


Funding Acknowledgements

KR thanks the Czech Science Foundation, grant number 14-22346S for funding. This work was partly supported by grants-in-aid from the Japan Society for the Promotion of Science (JSPS) to KS (No. 26291064) and HN (No. 26650106 and 15H01247). RM thanks the European Research Council, Grant number ERC 2011 StG 281659 (MeioSight), the Fondation Simone et Cino del Duca and the INRA BAP department. The IJPB benefits from the support of the Labex Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.