Journal article

AAA-ATPase FIDGETIN-LIKE 1 and Helicase FANCM Antagonize Meiotic Crossovers by Distinct Mechanisms

Chloe Girard, Liudmila Chelysheva, Sandrine Choinard, Nicole Froger, Nicolas Macaisne, Afef Lehmemdi, Julien Mazel, Wayne Crismani, Raphael Mercier

PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2015

Abstract

Meiotic crossovers (COs) generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1) as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both F..

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Grants

Awarded by European Community


Awarded by European Research Council


Funding Acknowledgements

This work has been funded by the European Community's Seventh Framework Programme FP7/2007-2013 [KBBE-2009-222883 (MeioSys)] and the European Research Council [ERC 2011 StG 281659 (MeioSight)]. Work in the Mercier laboratory is also supported by the Fondation Schlumberger pour l'education et la recherche (FSER) and the Fondation Simone et Cino del Duca / Institut de France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.