Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria
Catherine S Palmer, Jieqiong Lou, Betty Kouskousis, Elvis Pandzic, Alexander J Anderson, Yilin Kang, Elizabeth Hinde, Diana Stojanovski
JOURNAL OF CELL SCIENCE | COMPANY BIOLOGISTS LTD | Published : 2021
The mitochondrial inner membrane is a protein-rich environment containing large multimeric complexes, including complexes of the mitochondrial electron transport chain, mitochondrial translocases and quality control machineries. Although the inner membrane is highly proteinaceous, with 40-60% of all mitochondrial proteins localised to this compartment, little is known about the spatial distribution and organisation of complexes in this environment. We set out to survey the arrangement of inner membrane complexes using stochastic optical reconstruction microscopy (STORM). We reveal that subunits of the TIM23 complex, TIM23 and TIM44 (also known as TIMM23 and TIMM44, respectively), and the com..View full abstract
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Awarded by Australian Research Council (ARC)
Awarded by Australian National Health and Medical Research Council
D.S. is supported by the Research Fellowship from the Mito Foundation. We acknowledge funding from the Australian Research Council (ARC) (DP170101249 to D.S.). E.H. and J.L. are supported by grants from the Australian National Health and Medical Research Council (APP1124762 and APP1164000) and the ARC (DP180101387).