Curvature in the reproductive tract alters sperm-surface interactions
Mohammad Reza Raveshi, Melati S Abdul Halim, Sagar N Agnihotri, Moira K O'Bryan, Adrian Neild, Reza Nosrati
NATURE COMMUNICATIONS | NATURE RESEARCH | Published : 2021
The fallopian tube is lined with a highly complex folded epithelium surrounding a lumen that progressively narrows. To study the influence of this labyrinthine complexity on sperm behavior, we use droplet microfluidics to create soft curved interfaces over a range of curvatures corresponding to the in vivo environment. We reveal a dynamic response mechanism in sperm, switching from a progressive surface-aligned motility mode at low curvatures (larger droplets), to an aggressive surface-attacking mode at high curvatures (smaller droplets of <50 µm-radius). We show that sperm in the attacking mode swim ~33% slower, spend 1.66-fold longer at the interface and have a 66% lower beating amplitude ..View full abstract
Awarded by Australian Research Council (ARC)
This work was supported by the Australian Research Council (ARC) Discovery Project Grants (DP190100343 to R.N. and DP210103361 to A.N. and R.N.) and Monash Interdisciplinary Research Grants to R.N. This work was performed in part at the Melbourne Center for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). We thank D. Jo Merriner for her assistance with the sperm capacitation assay and Farin Yazdan Parast for her help with fluorescent imaging.