Journal article

238.Determination of differentially displayed oxygen-sensitive genes in bovine blastocysts

AJ Harvey, M Kirstein, A Navarrete-Santos, KL Kind, B Fischer, JG Thompson

Reproduction, Fertility and Development | CSIRO Publishing | Published : 2004

Abstract

Oxygen-regulated gene expression in the bovine embryo contrasts markedly with that observed in the mouse. Under low (2%) oxygen moderate changes in gene expression are observed in the bovine blastocyst, compared with 3- to 4-fold increases in the mouse. We have determined that these moderate gene expression changes are most likely regulated by Hypoxia-Inducible Factor (HIF)-2 transcription factor activity in the bovine, in the absence of HIF1, although HIF2 target genes are largely unknown. The aim of this study was to screen, by differential display RT-PCR, for putative oxygen-regulated transcripts that might confer developmental competence in blastocysts cultured under varying oxygen atmos..

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University of Melbourne Researchers